USPTO Patents Application 10687850

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2003 ^2 ^20 H (20.02.2003)

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COIN 33/543
PCT/JP02/08163
2002 ^8 fl9 a (09.08.2002)

(30) S5fe<tT-^:

!f#igl2001-242765 2001 ^8 ^ 9 B (09.08.2001) JP

(71) a3aAr*§*K^<±■c(7)^gg^lcol^r;: «^t®

^itS^^^ti: (MATSUSHITA ELECTRIC INDUS-
TRIAL CO., LTD.) [JP/JP]; T 571-8501 :*;BRJff PIKifJ
::*:¥Pia 1 O O 6 Osaka (JP).

(72) ^m%;tSJ;:XS

(75) 5§W%/tisilA r*§^-':5l^■ca)^;: lERI

(NADAOKA,Masataka) [JP/JP]; T 799-3113 S jfi

ff^rtr 8 1 9-5 Ehime (JP). SIS = ft
(TAKAHASHI,Mie) [JP/JP]; f 792-0026 ^
g/grfi ^SB90r2-1 3-1 Ehime (JP). ea*
(TANAKA,Hirotaka) [JP/JP]; T 791-1 102 ^Ul
m *tt»T 5 3 3-1-1 0 2 Ehime (JP). :ibfiS ICiK
(KJTAWAKI,Fumlhisa) [JP/JP]; =r 571-0064 :^B5Jfip PI
HrfT^^PT2 5-3 «i^^2 1 1 -^Osaka(JP).

(74) itmX: S- (HAYASE,Kenichi); =f532-0003 X

iT:*:IKe;n 3Pg^2g^#i^¥«iRSOsaka(jP).

(81) ft^g (^P^;: ON, JP, KR, US.

(84) figs (TStt;: 3 - □ »y / (AT, BE, BG. CH. CY,
CZ, DE, DK. EE, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL,
PT, SE, SK, TR).

(54) Title: BIOSENSORS AND MEASUREMENT METHOD

(54) ^mo^^i /Utiii^v-s BLXfm^um

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(57) Abstract: A biosensor provided with a development layer for developing a solution sample, and having a reagent part which
is immobilized on a part of the development layer and another reagent part which is held in a labeled and dry state on a part of
the development layer and can be eluted by developing the solution sample. By this biosensor, a component to be measured in the
solution sample is qualitatively or quantitatively measured by quantifying the bonded labeling reagent in the reagent immobilization
part In this biosensor, a prozone phenomenon can be detected by using a plural number of the reagent immobilization parts and
varying the aflflnities of the respective reagent immobilization parts for the subject to be measured in the solution sample or the
labeling reagent Also, a biosensor having a high measurement accuracy whereby the concentration of the subject to be measured
in the solution sample can be measured over a broadened dynamic range is provided. Thus, it is possible to provide a biosensor by
which a prozone phenomenon can be detected even in case of measuring a subject concentration over a wide range and which has a
wide <fynamic measurement range, a high precision and a high accuracy.

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INTERNATIONAL SEARCH REPORT

Inremational application No.

PCT/JP02/08163

A. CLASSIFICATION OF SUBJECT MATTER
Int.Cl'' G01N33/543

According to International Patent Classification (IPC) or to both nationa] classification and IPC

B. FIELDS SEARCHED

Minimum documentation searched (classification system followed by classification symbols)
Int.Cl^ G01N33/543

Documentation searched other than minimum documentation to the extent that such documents are included in the fields searched
Jitsuyo Shinan Kbho 1922-1996 Toroku Jitsuyo Shinan Koho 1994-2002

Kbkai Jitsuyo Shinan Koho 1971-2002 Jitsuyo Shinan Toroku Kbho 1996-2002

Electronic data base consulted during the international search (name of data base and, where practicable, search terms used)

C. DOCUMENTS CONSIDERED TO BE RELEVANT

Category*

. Citation of document, with indication, where appropriate, of the relevant passages

Relevant to claim No.

X

JP 10-185921 A (Wakunaga Pharmaceutical Co., Ltd.)/
14 July, 1998 (14.07-98),

(Family: none)

1,2,4,5,
8-22,25-30

A

JP 8-278305 A (Nippon Shoji Kaisha, Ltd.),
22 October, 1996 (22.10.96),
(Family: none)

1-31

A

JP 11-326325 A (Dainippon Printing Co., Ltd.), ■
26 November, 1999 (26.11.99),
(Family: none)

1-31

A

JP 2000-46831 A (Bayer Corp.),
18 February, 2000 (18.02.00),
& EP 987551 A

^ 1-31

I [ Further documents are h'sted in the continuation of Box C. | [ See patent family annex.

♦ Special categories of cited documents: **V later document published after the international filing date or

"A" document defining the general state of the art which is not priority date and not in conflict with the application but cited to

considered to be of particular relevance understand the principle or theory underlying the invention

"E" earlier document but published on or after the international filing "X" document of particular relevance; the claimed invention cannot be

date considered novel or cannot be considered to involve an inventive

document which may throw doubts on priority claim(s) or which is step when the document is taken alone

cited to establish the publication date of another citation or other "Y^ document of particular relevance; the claimed invention cannot be

special reason (as specified) considered to involve an inventive step when the document is

"0^ document referring to an oral disclosure, use, exhibition or other combined with one or more other such documents, such

means combination being obvious to a person skilled in the art

*V document ptiblished prior to the international filing date but later document member of the same patent family

than the priority date claimed

Date of the actual completion of the international search
08 November, 2002 (08.11.02)

Date of mailing of the international search report

26 November, 2002 (26.11.02)

Name and mailing address of the ISA/

Japanese Patent Office

Facsimile No.

Authorized officer
Telephone No.

Form PCT/ISA/210 (second sheet) (July 1998)

ffl^^s#e m^mms'^ p ct/ j p o 2/0 s 1 e 3

A. ?sm(o&i-i>i^woi^m (ii§g#rF^« (i po )

Int. C 1 ' GO 1N3 3/543

Int. CI' GO INS 3/543

H*ffl*^?^^<&$8 1922-1996<¥

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X

JP 10-185921 A mikmmm^^m

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1, 2, 4, 5, 8-
22. 25-30

A

JP 8-278305 A ( H «*:^S;^^±)
1 9 9 6. 1 0. 2 2 (77^

1-31

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